Fig. 5.

Western blot analysis of phage H binding to PEDV. Sedimented PEDV was subjected to SDS-PAGE followed by Western blot on nitrocellulose (NC) membranes. The NC membranes were sliced and incubated with (1) phage H, (2) anti-PEDV S polyclonal antibody, (3) M13 phage library, or control phages bearing the peptides (4) SVSVGMKPSPRP or (5) MSCNDTLCLLPN. The membranes were successively incubated with anti-M13 polyclonal antibody and HRP-conjugated goat anti-rabbit IgG. The protein bands were visualized using 3,3׳-diaminodbenzidine. The sizes of protein markers and location of the PEDV S protein are indicated.