Fig. 3.

Cell cycle regulation effects in different strains and different cell line. (A) Cells were mock-infected or infected with Herts/33 (NDV) or La Sota (NDV) at an MOI of 1. At 24 h post-infection, the cells were pulsed with 10 μM BrdU for 1 h prior to harvest and then collected by trypsinization. The cells were fixed, stained with anti-BrdU antibody and propidium iodide, and analyzed by flow cytometry. (B) Representative histograms of flow cytometry data are shown. Statistical significance was determined by comparisons between mock-infected and Herts/33-infected or La Sota-infected. (C) DF-1 cells and HeLa cells were mock-infected or infected with Herts/33 (NDV) at 1of MOI. At 24 h post-infection, cells were pulsed with 10 μM BrdU for 1 h prior to harvest and then collected by trypsinization. The cells were fixed, stained with anti-BrdU antibody and propidium iodide, and analyzed by flow cytometry. (D) Representative histograms of flow cytometry data are shown. Statistical significance was determined by comparisons between DF-1 cells and HeLa cells. Data are presented as mean ± SD for three independent experiments. *P < 0.05.