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. 2019 Nov 7;540:45–56. doi: 10.1016/j.virol.2019.11.007

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© 2019 Elsevier Inc.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

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Fig. 6 — Construction of the N-deficient PEDV infectious clone, pPEDV-mCherry-ΔN. (A) Schematic representation of a frameshift mutation strategy to silence N expression from the pPEDV-mCherry-derived infectious clone to yield pPEDV-mCherry-ΔN. CMV, cytomegalovirus immediate-early promoter; HDV, hepatitis delta virus ribozyme self-cleavage site; BGH, bovine growth hormone termination; T, transcription terminator. (B) Western blot analysis of lysates prepared from HEK293T cells transfected with pPEDV-mCherry or pPEDV-mCherry-ΔN (2 μg each) at 72 hpt.