Fig. 3.

Changes in Toll/Interleukin-1 receptor (TIR), myeloid differentiation primary response gene 88 (MyD88), and Nuclear factor (NF)-κB genes mRNA expression induced by porcine epidemic diarrhea virus (PEDV) non S-INDEL and PEDV S-INDEL strains in intestinal mucosa (A-D). Ten pigs in each group were infected with PEDV non S-INDEL, PEDV S-INDEL or media (negative control) and five pigs from each group were necropsied at 3 and 7 days post-infection (dpi). The mRNA levels of TRIF (A), NF-κB (B), MyD88A (C), and MyD88B (D) at the intestinal mucosa was determined individually in each animal by SYBR- green qRT-PCR. All samples were tested in triplicate and the results are expressed as fold changes relative to the control animals Data are presented as means ± standard errors. Significant difference between PEDV S-INDEL PEDV non S-INDEL and control group are expressed with their p values. *p < 0.05; **p < 0.01, ***p < 0.001.