Fig. 5.

Changes in the nuclear translocator NF-κB transcriptional factor family, NF-κB1 (p50) and RelA (p65) genes mRNA expression induced by porcine epidemic diarrhea virus (PEDV) non S-INDEL and PEDV S-INDEL strains in intestinal mucosa (A-B). Ten pigs in each group were infected with PEDV non S-INDEL, PEDV S-INDEL or media (negative control) and five pigs from each group were necropsied at 3 and 7 days post-infection (dpi). The mRNA levels of NF-κB1 (p50) (A), and RelA (p65) (B) at the intestinal mucosa was determined individually in each animal by SYBR-green qRT-PCR. All samples were tested in triplicate and the results are expressed as fold changes relative to the control animals Data are presented as means ± standard errors. Significant difference between PEDV S-INDEL PEDV non S-INDEL and control group are expressed with their p values. *p < 0.05; **p < 0.01, ***p < 0.001.