. 2017 Dec 14;517:188–198. doi: 10.1016/j.virol.2017.11.024

Table 1.

Primers used for real-time PCR analysis of genes expression of pattern-recognition receptor TLR and RIG-I-like and downstream mediators on pig intestinal mucosa.

Gene Name	Primer Name	Primer Sequence (5’−3”)	Amplicon Length	Accession number
GAPDH	Por-GAPDH-40F	GGAAAGGCCATCACCATCTT	85 bp	XM_021091114.1
GAPDH	Por-GAPDH−125R	CATGGTCGTGAAGACACCAG	85 bp	XM_021091114.1
Β-Actin	Por-βActin−598 F	CCCAGCACCATGAAGATCAA	88 bp	XM_005670976.2
Β-Actin	Por-βActin−686 R	GATCCACATCTGCTGGAAGG	88 bp	XM_005670976.2
TLR2	Por-TLR2-1601F	GAGTCTGCCACAACTCAAAGA	68 bp	XM_005653576.3
TLR2	Por-TLR2–1669R	CAGAACTGACAACATGGGTAGAA	68 bp	XM_005653576.3
TLR3	Por-TLR3-1658F	GCGGTCCTGTTCAGTTTCT	72 bp	KT735340.1
TLR3	Por-TLR3–1730R	AAGGCATCTGCTGGGATTT	72 bp	KT735340.1
TLR4	Por-TLR4-263F	AACTGCAGGTGCTGGATTTAT	74 bp	AB078418.1
TLR4	Por-TLR4–337R	CCGTCAGTATCAAGGTGGAAAG	74 bp	AB078418.1
TLR7	Por-TLR7-1376F	CCCAGGTCCTCGAATCATTAC	77 bp	DQ647699.2
TLR7	Por-TLR7–1453R	CATTAAGAGGCAAGGAGGAAGA	77 bp	DQ647699.2
TLR8	Por-TLR8-1980F	CTTTGATGATGACGCTGCTTTC	77 bp	KF019635.1
TLR8	Por-TLR8–2057R	GGTGTGTCACTCCTGCTATTC	77 bp	KF019635.1
TLR9	Por-TLR9-597F	CCTCACACATCTCTCACTCAAG	82 bp	KC860785.1
TLR9	Por-TLR9–679R	GGTGACAATGTGGTTGTAGGA	82 bp	KC860785.1
RIG-I	Por_RIG-I_1922F	GAGCCCTTGTGGATGCTTTA	91 bp	KC011279.1
RIG-I	Por_ RIG-I_2013R	GGGTCATCCCTATGTTCTGATTC	91 bp	KC011279.1
TRIF	Por_TRIF_1027F	CTCCGGTGCAGTCAAACA	91 bp	KC969185.1
TRIF	Por_TRIF_1118R	GGTAGTGTGTGCTGGTTTCT	91 bp	KC969185.1
MyD88A	Por_MyD88_1852F	GGCAGCTGGAACAGACCAA	41 bp	EU056736.1
MyD88A	Por_ MyD88_1893R	GGCAGGACATCTCGGTCAGA	41 bp	EU056736.1
MyD88B	Por_MyD88_2501F	TGCAGGTGCCCATCAGAAG	64 bp	EU056737.1
MyD88B	Por_ MyD88_2565R	TGATGAACCGCAGGATGCT	64 bp	EU056737.1
NF-ƙB1 (p105)	Por_ NF-ƙB1_1387F	GAGGTGCATCTGACGTATTC	118 bp	NM_001048232.1
NF-ƙB1 (p105)	Por_ NF-ƙB1_1505R	CACATCTCCTGTCACTGCAT	118 bp	NM_001048232.1
NF-ƙB1 (p50)	Por_ NF-ƙB1_1249F	AAGCACGGAACTGTAGACAC	107 bp	KC316024.1
NF-ƙB1 (p50)	Por_NF-ƙB1_1355R	TCTGTGGTTTCTGTGACTTTCC	107 bp	KC316024.1
RELA (p65)	Por_RELA_1616F	ACATGGACTTCTCAGCCCTTCTGA	285 bp	CN155798.1
RELA (p65)	Por_RELA_1331R	CCGAAGACATCACCCAAAGATGCT	285 bp	CN155798.1
IRF7	Por_IRF−7_1193F	CACTACACAGAGAAGCTGCT	91 bp	HQ026022.1
IRF7	Por_IRF−7_1284R	ACCTCCCAGTAGACTTTGC	91 bp	HQ026022.1
TRAF6	Por_TRAF−6_1338F	GGGAACGATACGCCTTACAA	156 bp	NM_001105286.1
TRAF6	Por_TRAF−6_1494R	CTCTGTCTTAGGGCGTCC	156 bp	NM_001105286.1
IL−6	Por_IL−6_260F	CAAGGAGGTACTGGCAGAAA	164 bp	JQ839263.1
IL−6	Por_IL−6_424F	CAGCCTCGACATTTCCCTTAT	164 bp	JQ839263.1
IL12-p35	Por_IL12-p35_200F	CAGGCCCAGGAATGTTCAAA	166 bp	NM_213993.1
IL12-p35	Por_IL12-p35_366R	CGTGGCTAGTTCAAGTGGTAAG	166 bp	NM_213993.1
TNF-α	Por_TNFα_157F	CCTACTGCACTTCGAGGTTATC	158 bp	JF831365.1
TNF-α	Por_TNFα_315R	GCATACCCACTCTGCCATT	158 bp	JF831365.1
IFN-α	Por_IFNα_372F	TTCTGCACTGGACTGGATC	103 bp	KF414740.1
IFN-α	Por_IFNα_475R	TCTGTGGAAGTATTTCCTCACAG	103 bp	KF414740.1

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH); Β-Actin; Toll-like receptor (TLR); retinoic acid-inducible gene-1 (RIG-1)-like receptor; TIR-domain-containing adapter-inducing interferon-β (TRIF); myeloid differentiation primary response gene 88 (MyD88); nuclear factor (NF)-κB; nuclear factor NF-kappa-B p65 subunit (RELA); interferon regulatory transcription factor (IRF); TNF receptor associated factor (TRAF); interleukin (IL); tumor necrosis factor (TNF); Interferons (IFN).