Fig. 1.

Experimental workflow. OL/BDV cells and control cells were maintained in SILAC medium. An aliquot of crude proteins from the mixed cells was digested in solution by trypsin. Protein identification and quantification were performed using LC-MS/MS and data inquiry. In parallel, histones from the cell mixture were extracted, digested, and affinity enriched followed by LC-MS/MS analysis. Further biochemistry was applied to validate the MS analysis results.