Fig. 3.

Differences in EAV, MERS-CoV and HCoV-229E sensitivity to cyclophilin A depletion. A) Huh7-CypA^KO cell clones #1 and #2 were analyzed for lack of CypA expression by Western blot analysis. Beta-actin was used as loading control. B, D-E) Parental Huh7 cells and Huh7-CypA^KO clones #1 and #2 were infected with MERS-CoV (B), HCoV-229E (D), or EAV (E) at an MOI of 0.01. (C) Parental Huh7 cells and Huh7-CypA^KO clone #2 were infected with MERS-CoV at an MOI of 5. Virus yields at 48 h p.i. (B,D), 24 h p.i. (C) or 32 h p.i. (E) were determined by plaque assay. Results are the mean ± SD of triplicate harvests from a representative experiment. F) Comparison of EAV progeny yields in Huh7-CypA^KD (Chatterji et al., 2009), Huh7-CypA^KO,pool cells (used in Fig. 1), and Huh7-CypA^KO cell clone #1 (used in Fig. 3), presented as the difference in virus yields between Huh7 control cells and CypA^KO or CypA^KD cells (in log₁₀ reduction in EAV titer). Results are the mean ± SD from two independent experiments.