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. 2020 Mar 25;11:431. doi: 10.3389/fimmu.2020.00431

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Copyright © 2020 Grødeland, Baranowska-Hustad, Abadejos, Blane, Teijaro, Nemazee and Bogen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Vaccination and protection against challenge with H7N1 influenza. (A) BALB/c mice (n = 10 mice/group) were immunized on days 0 and 28, as indicated by arrows, and sera analyzed for development of IgG responses against recombinant HA from PR8 (H1N1), A/Hong Kong/1/1968 (H3N2), A/Hong Kong/483/97 (H5N1), A/Shanghai/1/2013 (H7N9), and A/Hong Kong/1073/99 (H9N2). Values given are mean ± SEM. *p < 0.05 as compared to NaCl (two-way ANOVA and Bonferroni post-test). (B) At day 49 after vaccination, mice were challenged with 5 × LD50 of influenza A/turkey/Italy/3889/1999 (H7N1) and monitored for weight (B) and survival (C).