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. 2020 Feb 6;61(4):546–559. doi: 10.1194/jlr.D119000388

Fig. 1.

Fig. 1.

Association of largeBiT-LPL with smallBiT-GPIHBP1. A: Schematic of the NanoBiT LPL binding assay. B: SmallBiT-GPIHBP1-expressing RHMVECs were grown to confluence. Luminescent substrate (Nano-Glo® live cell substrate) was added to cells and luminescence was read every minute. After 6 min (dotted line), the indicated concentrations of largeBiT-LPL or largeBiT-EL were added to each well and luminescence continued to be measured each minute for an additional 30 min. Points represent mean ± 95% CI of four independent experiments (n = 6 per group per experiment). C: Western blot of the LargeBiT LPL (LPL-LgBiT)- and LargeBiT-EL (EL-LgBiT)-conditioned media used in B. D: Western blot of the LargeBiT LPL (LPL)- and LargeBiT-LPL C445Y (mutLPL)-conditioned media used in E. E: SmallBiT-GPIHBP1-expressing RHMVECs were grown to confluence. Luminescent substrate was added to cells and luminescence was read every minute. After 6 min (dotted line), the indicated concentrations of largeBiT-LPL (LPL) or largeBiT-LPL C445Y (mutLPL) were added to each well and luminescence continued to be measured each minute for an additional 30 min. Points represent mean ± 95% CI of three independent experiments (n = 6 per group per experiment).