Skip to main content
. 2020 Feb 6;61(4):546–559. doi: 10.1194/jlr.D119000388

Fig. 2.

Fig. 2.

Disruption of LPL-GPIHBP1 binding by heparin. A: Schematic of the NanoBiT LPL dissociation assay. B: Western blot of lysates of smallBiT-GPIHBP1-expressing RHMVECs incubated with LargeBiT-LPL for 3.5 h at 4°C, washed, and then treated with or without 100 U/ml heparin for 30 min. C, D: SmallBiT-GPIHBP1-expressing endothelial cells were incubated with LargeBiT-LPL for 2 h at 4°C and washed. Luminescent substrate was added to cells and luminescence read every 3 min for 15 min. After 15 min 100 U/ml heparin (+heparin) or water (–heparin) were added to the samples and luminescence was read every 3 min for an additional 15 min. C: Luminescent signal over time (maximum signal for each sample set to 100%). D: Luminescence signal over time normalized to the –heparin control at each time point. Points represent mean ± 95% CI of three independent experiments (n = 3–6 per group per experiment).