Fig. 4.

Steady state levels of total d18:1 sphingoid base lipids were measured in the oligodendrocytes isolated from the brains of postnatal rats of various ages. LC-MS/MS was used to quantitate the mass levels of total d18:1 ceramides (CER), d18:1 SMs, d18:1 GluCERs, d18:1 GalCERs, and d18:1 sulfatides (Sulfatide) in both the primary oligodendrocytes isolated from the brain tissue of postnatal rats using the Percoll gradient method as described in the Isolation of primary oligodendrocytes from rat brain section of the Materials and Methods. Data are graphed as picomoles of lipid per 100 μg of total protein. Samples are from pooled oligodendrocytes derived from 20 rat pups.