Fig. 6.

mRNA expression levels of the SPT large and small subunits are dynamically regulated in the developing rat brain. Total RNA was isolated from the brains of postnatal rats of various ages as indicated in each panel. RT-qPCR analysis was performed and mRNA expression levels of the large subunits, SPTLC1 (A), SPTLC2 (B), and SPTLC3 (C), as well as the small subunits, ssSPTa (D) and ssSPTb (E), were quantitated. Data have been normalized to GAPDH expression levels and then set relative to day 2. Data are presented as mean ± SEM, n = 5 animals per age group.