Fig. 7.

mRNA expression levels of the three ORMDL isoforms are dynamically regulated in the developing rat brain. Total RNA was isolated from the brains of postnatal rats of various ages as indicated in each panel. RT-qPCR analysis was performed and mRNA expression levels of ORMDL1 (A), ORMDL2 (B), and ORMDL3 (C), as well as the housekeeping gene, hypoxanthine phosphoribosyltransferase (HPRT) (D), were quantitated. Data have been normalized to GAPDH expression levels and then set relative to day 2. Data are expressed as mean ± SEM, n = 5 animals per age group.