Fig. 4.
Metabolic analysis of VLCFA-CoA species using FA D4-26:0. Metabolic profiles of 26:0- and 26:1-CoA species containing a deuterium-labeled (A) or nonlabeled (B) acyl moiety in ABCD1-KO (#1) and WT HeLa cells (WT). Cells were cultured in medium containing 10% FBS and were treated with 30 μM of FA D4-26:0 and the MβCD complex. Cells were harvested at 1, 3, 5, 8, and 24 h after treatment. We confirmed that the retention times of each deuterium-labeled acyl-CoA species were almost identical with those of the corresponding nonlabeled VLCFA-CoA species. The MRM transitions (Q1/Q3) used are indicated in each panel. Data represent the mean ± SD, n = 3. Statistical analyses were performed with the Student’s t-test; *P < 0.05, **P < 0.01, ***P < 0.001 for ABCD1-KO versus WT HeLa cells