Fig. 5.
Metabolic analysis of VLCFA-CoA species using FA D2-18:1. Metabolic profiles of each deuterium-labeled 18:1- and 26:1-CoA species (A) and nonlabeled 18:1- and 26:1-CoA species (B) were examined in ABCD1-KO (#1) and WT HeLa cells. The metabolic profiles of the other labeled and nonlabeled acyl-CoA species are depicted in supplemental Fig. S3. Cells were cultured in medium containing 10% FBS and treated with 30 μM of FA D2-18:1 and the MβCD complex. The cells were harvested at 1, 3, 5, 8, and 24 h after treatment. The MRM transitions (Q1/Q3) used are indicated in each panel. Statistical analyses were performed with the Student’s t-test; *P < 0.005, **P < 0.01, ***P < 0.001 for ABCD1-KO versus WT HeLa cells