Fig. 6.
Pulse-chase analysis of VLCFA-CoA species using FA D2-18:1. Metabolic profiles of 15 acyl-CoA species containing a deuterium-labeled (A) or nonlabeled (B) acyl moiety in ABCD1-KO (#1) and WT HeLa cells were examined. Cells were cultured in medium containing 10% FBS and treated with 30 μM of FA D2-18:1 and the MβCD complex for an hour, after which the medium was replaced with fresh medium containing 10% FBS. The cells were then harvested at 1, 2, 4, 6, and 24 h after treatment. Acyl-CoA species were extracted and analyzed by LC-MS/MS analysis. The MRM transitions (Q1/Q3) used are indicated in each panel. Statistical analyses were performed with Student’s t-test; *P < 0.05, **P < 0.001, ***P < 0.001 for ABCD1-KO versus WT HeLa cells