TABLE 1.
Calibration curve for the acyl-CoA species
| Linearity | Precision [CV(%)]a | Accuracy (%) | ||||||||||
| Compound | Range (pmol) | Weight | Slope | Intercept | r2 | QC-Lb | QC-Mb | QC-Hb | QC-Lb | QC-Mb | QC-Hb | |
| 17:0-CoA/(D31)-16:0-CoA | 0.05–100 | 1/x2 | 0.061 | −0.0024 | 0.991 | Intra-day (n = 12) | 10.6 | 6.3 | 3.7 | 9.5 | 5.2 | 5.8 |
| Inter-day (n = 4+4+4) | 21.2 | 10.3 | 6.3 | 21.2 | 11.8 | 9.2 | ||||||
a
Precision was calculated as the coefficient of variation (CV).
b
Three samples with 0.050, 1.0, and 10 pmol of 17:0-CoA were mixed with 10 pmol of (D31)-16:0-CoA per injection and were analyzed as QC compounds QC-L (low), QC-M (middle), and QC-H (high), respectively.