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. 2020 Feb 19;61(4):523–536. doi: 10.1194/jlr.P119000325

TABLE 2.

Quantification of the acyl-CoA species in the X-ALD fibroblasts

CCALD (n = 4) AMN (n = 3) Control (n = 5)
Signals Q1 (m/z) Q3 (m/z) Retention Time (min) Amounta Percent Amounta Percent Amounta Percent
14:0-CoA 978 471 0.97 18.3 1.6 13.9 1.9 15.5 2.3
15:0-CoA 992 485 1.12 3.2 0.3 0.6 0.1 1.9 0.3
16:0-CoA 1,006 499 1.34 102.0 8.9 48.6 6.5 71.4 10.5
16:1-CoA 1,004 497 1.06 28.8 2.5 16.3 2.2 22.5 3.3
17:0-CoA 1,020 513 1.62 25.5 2.2 18.2 2.4 23.8 3.5
17:1-CoA 1,018 511 1.24 8.4 0.7 4.3 0.6 5.0 0.7
18:0-CoA 1,034 527 2.07 31.8 2.8 15.6 2.1 21.4 3.2
18:1-CoA 1,032 525 1.48 211.0 18.5 108.3 14.5 135.2 19.9
18:2-CoA 1,030 523 1.21 23.9 2.1 15.4 2.1 18.5 2.7
18:3-CoA 1,028 521 1.05 6.6b 0.6 2.7 0.4 3.7 0.5
19:1-CoA 1,046 539 1.79 3.3 0.3 n.q. 0.0 1.1 0.2
19:2-CoA 1,044 537 1.40 1.3 0.1 n.q. 0.0 0.8 0.1
19:4-CoA 1,040 533 1.30 9.4 0.8 8.8 1.2 12.5 1.8
20:0-CoA 1,062 555 3.56 3.2 0.3 0.4 0.1 0.9 0.1
20:1-CoA 1,060 553 2.25 9.9 0.9 5.5 0.7 5.9 0.9
20:2-CoA 1,058 551 1.64 12.5c 1.1 6.1 0.8 7.8 1.1
20:3-CoA 1,056 549 1.36 74.7 6.5 43.8 5.9 54.9 8.1
20:4-CoA 1,054 547 1.19 109.3 9.6 82.1 11.0 85.8 12.6
20:5-CoA 1,052 545 1.03 18.5 1.6 7.5 1.0 12.1 1.8
21:0-CoA 1,072 565 1.27 n.q. 0.0 0.6 0.1 1.0 0.1
21:3-CoA 1,066 559 1.66 1.8b 0.2 0.5 0.1 0.2 0.0
22:0-CoA 1,090 583 5.28 5.1b 0.4 4.2 0.6 1.5 0.2
22:1-CoA 1,088 581 3.75 8.0 0.7 6.9 0.9 6.3 0.9
22:2-CoA 1,086 579 2.67 1.4 0.1 0.4 0.0 0.6 0.1
22:3-CoA 1,084 577 2.00 7.8 0.7 3.7 0.5 6.7 1.0
22:4-CoA 1,082 575 1.62 50.0 4.4 18.0 2.4 31.6 4.6
22:5-CoA 1,080 573 1.35 64.3c 5.6 32.0 4.3 46.5 6.8
22:6-CoA 1,078 571 1.20 22.2 1.9 11.5 1.5 16.8 2.5
24:0-CoA 1,118 611 6.46 10.8b 0.9 16.7 2.2 0.9 0.1
24:1-CoA 1,116 609 5.32 33.6b 2.9 33.4b 4.5 12.9 1.9
24:2-CoA 1,114 607 4.40 2.7 0.2 1.3 0.2 0.8 0.1
24:3-CoA 1,112 605 3.21 3.7 0.3 1.8 0.2 3.2 0.5
24:4-CoA 1,110 603 2.40 5.3 0.5 1.6b 0.2 8.7 1.3
24:5-CoA 1,108 601 1.94 7.5c 0.7 3.6b 0.5 6.5 1.0
24:6-CoA 1,106 599 1.62 2.1 0.2 1.0 0.1 1.3 0.2
25:1-CoA 1,130 623 5.92 15.2 1.3 16.6 2.2 9.2 1.4
26:0-CoA 1,146 639 7.50 0.9 0.1 1.6 0.2 n.q. 0.0
26:1-CoA 1,144 637 6.44 132.1b 11.6 134.7d 18.1 17.9 2.6
26:2-CoA 1,142 635 5.79 15.3b 1.3 11.2 1.5 3.6 0.5
26:5-CoA 1,136 629 2.96 2.3 0.2 n.q. 0.0 1.6 0.2
27:1-CoA 1,158 651 6.99 11.7d 1.0 10.9d 1.5 0.2 0.0
28:1-CoA 1,172 665 7.49 26.0b 2.3 24.1b 3.2 0.1 0.0
28:2-CoA 1,170 663 6.72 7.2b 0.6 6.5 0.9 0.2 0.0
28:6-CoA 1,162 655 6.07 n.q. 0.0 1.1 0.2 0.2 0.0
29:2-CoA 1,184 677 6.52 1.6 0.1 1.3 0.2 0.5 0.1
Others — — — 3.2 0.3 0.9 0.1 0.3 0.0
Total — — — 1,143.4b 100.0 744.3 100.0 680.7 100.0
VLCFA-CoA (DB = 0)e 11.7 1.0 18.2 2.4 0.9 0.1
VLCFA-CoA (DB = 1)e 218.6b 19.1 219.8d 29.5 40.5 5.9
VLCFA-CoA (DB = 2)e 26.8b 2.3 20.3b 2.7 5.0 0.7
VLCFA-CoA (DB = 3)e 3.7 0.3 1.8 0.2 3.2 0.5
VLCFA-CoA (DB = 4)e 6.0 0.5 1.6b 0.2 8.7 1.3
VLCFA-CoA (DB = 5)e 9.8 0.9 3.6 0.5 8.1 1.2
VLCFA-CoA (DB = 6)e 2.8 0.2 2.3 0.3 1.9 0.3
Total of VLCFA-CoA 279.4b 24.4 267.5d 35.9 68.3 10.0
a

Expressed as picomoles per milligram of protein. The ratio of peak area for each acyl-CoA/D31-16:0-CoA was used to calculate the amount of each acyl-CoA species. n.q., below the quantitation range.

b

P < 0.05 versus control (Dunnett T3 post hoc test).

c

P < 0.05 versus AMN (Dunnett T3 post hoc test).

d

P < 0.01 versus control (Dunnett T3 post hoc test).

e

The total amount of each VLCFA-CoA species that contain very long-chain fatty acyl moieties with the number of double bonds (DBs) as indicated.