TABLE 3.
Structure of the PL species present in quantities significantly higher in the X-ALD fibroblasts
| Amountc | |||||||||||
| Signalsa,b | CCALD | AMN | Control | d | sn-1 FA (m/z)e | sn-2 FA (m/z)e | 1-Acyl LPC (m/z)e | Molecular Species | N-FA (m/z)e | SPC (m/z)e | Molecular Species |
| PC 32:0 | 5,625f,g | 3,939 | 3,741 | 778 | 18:0 (283) | 14:0 (227) | LPC 18:0 (508) | PC 18:0/14:0 | — | — | — |
| 16:0 (283) | 16:0 (227) | LPC 16:0 (480) | PC 16:0/16:0 | — | — | — | |||||
| PC 32:2 | 883 | 767f | 640 | 774 | 16:0 (255) | 16:2 (251) | LPC 16:0 (480) | PC 16:0/16:2 | — | — | — |
| 16:1 (253) | 16:1 (253) | LPC 16:1 (478) | PC 16:1/16:1 | — | — | — | |||||
| 14:0 (227) | 18:2 (279) | LPC 14:0 (452) | PC 14:0/18:2 | — | — | — | |||||
| PC 34:1 | 10,675h | 8,289 | 6,469 | 804 | 16:0 (255) | 18:1 (281) | LPC 16:0 (480) | PC 16:0/18:1 | — | — | — |
| PC 36:1 | 8,808f | 8,009 | 5,762 | 832 | 18:0 (283) | 18:1 (281) | LPC 18:0 (508) | PC 18:0/18:1 | — | — | — |
| 20:1 (309) | 16:0 (255) | LPC 20:1 (534) | PC 20:1/16:0 | — | — | — | |||||
| PC 36:4 | 4,751f | 4,246 | 3,212 | 826 | 16:0 (255) | 20:4 (303) | LPC 16:0 (480) | PC 16:0/20:4 | — | — | — |
| 18:2 (279) | 18:2 (279) | LPC 18:2 (504) | PC 18:2/18:2 | — | — | — | |||||
| PC 38:4 | 5,507f | 4,998 | 3,812 | 854 | 18:0 (283) | 20:4 (303) | LPC 18:0 (508) | PC 18:0/20:4 | — | — | — |
| PC 40:1 | 28f | 42 | 18 | 888 | 16:0 (255) | 24:1 (365) | LPC 16:0 (480) | PC 16:0/24:1 | — | — | — |
| PC 42:1 | 36f | 58 | 12 | 916 | 26:1 (393) | 16:0 (255) | LPC 26:1 (618) | PC 26:1/16:0 | — | — | — |
| 24:0 (367) | 18:1 (281) | LPC 24:0 (592) | PC 24:0/18:1 | — | — | — | |||||
| PC 42:2 | 29f | 45 | 14 | 914 | 24:0 (367) | 18:2 (279) | LPC 24:0 (592) | PC 24:0/18:2 | — | — | — |
| 24:1 (365) | 18:1 (281) | LPC 24:1 (590) | PC 24:1/18:1 | — | — | — | |||||
| PC 42:3 | 21f | 29 | 13 | 912 | 24:2 (363) | 18:1 (281) | LPC 24:2 (588) | PC 24:2/18:1 | — | — | — |
| PC 44:1 | 30f | 45 | 1 | 944 | 26:0 (395) | 18:1 (281) | LPC 26:0 (620) | PC 26:0/18:1 | — | — | — |
| PC 44:2 | 23f | 38 | 3 | 942 | 26:1 (393) | 18:1 (281) | LPC 26:1 (618) | PC 26:1/18:1 | — | — | — |
| PE 34:4 | 83 | 106 | 67 | 710 | 14:0 (227) | 20:4 (303) | LPE 14:1 (424) | PE 14:0/20:4 | — | — | — |
| SM 32:1 | 1,504 | 1,563h | 1,135 | 719 | — | — | — | 16:0 (255) | d16:1 (421) | SM d16:1/16:0 | |
| — | — | — | 14:0 (255) | d18:1 (449) | SM d18:1/14:0 | ||||||
| SM 34:1 | 20,208h | 13,219 | 11,769 | 747 | — | — | — | 16:0 (255) | d18:1 (449) | SM d18:1/16:0 | |
| SM 34:2 | 2,295 | 3,139f | 2,099 | 745 | — | — | — | 16:0 (255) | d18:2 (447) | SM d18:2/16:0 | |
| SM 44:1 | 248f | 193h | 42 | 887 | — | — | — | 26:0 (395) | d18:1 (449) | SM d18:1/26:0 | |
| SM 44:2 | 421 | 356f | 154 | 885 | — | — | — | 26:1 (393) | d18:1 (449) | SM d18:1/26:1 | |
Each PL species observed by semi-quantitative analysis is represented by the total carbon and double bond number of two fatty acyl moieties (PC) or by the long-chain base and an N-acyl moiety (SM).
PC 38:0 and SM 44:3 could not be assigned because of the absence of a spectrum corresponding to 1-acyl LPC and N-acyl moiety in LC-MS3 analysis, respectively.
The quantity of each PL species in the CCALD, AMN, and control fibroblasts picomoles per milligram of protein).
The m/z of the first precursor ion was used to analyze the structure of each PL species in MS3 using the negative ion mode.
Notation in bold indicates fatty acyl moieties with no less than 24 carbons. FA, LPC, and SPC represent fatty acyl-moiety, LPC, and sphingosylphosphorylcholine (SPC), respectively.
P < 0.05 versus control (Dunnett T3 post hoc test).
P < 0.05 versus AMN (Dunnett T3 post hoc test).
P < 0.01 versus control (Dunnett T3 post hoc test).