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. 2020 Feb 19;61(4):523–536. doi: 10.1194/jlr.P119000325

TABLE 3.

Structure of the PL species present in quantities significantly higher in the X-ALD fibroblasts

Amountc
Signalsa,b CCALD AMN Control d sn-1 FA (m/z)e sn-2 FA (m/z)e 1-Acyl LPC (m/z)e Molecular Species N-FA (m/z)e SPC (m/z)e Molecular Species
PC 32:0 5,625f,g 3,939 3,741 778 18:0 (283) 14:0 (227) LPC 18:0 (508) PC 18:0/14:0
16:0 (283) 16:0 (227) LPC 16:0 (480) PC 16:0/16:0
PC 32:2 883 767f 640 774 16:0 (255) 16:2 (251) LPC 16:0 (480) PC 16:0/16:2
16:1 (253) 16:1 (253) LPC 16:1 (478) PC 16:1/16:1
14:0 (227) 18:2 (279) LPC 14:0 (452) PC 14:0/18:2
PC 34:1 10,675h 8,289 6,469 804 16:0 (255) 18:1 (281) LPC 16:0 (480) PC 16:0/18:1
PC 36:1 8,808f 8,009 5,762 832 18:0 (283) 18:1 (281) LPC 18:0 (508) PC 18:0/18:1
20:1 (309) 16:0 (255) LPC 20:1 (534) PC 20:1/16:0
PC 36:4 4,751f 4,246 3,212 826 16:0 (255) 20:4 (303) LPC 16:0 (480) PC 16:0/20:4
18:2 (279) 18:2 (279) LPC 18:2 (504) PC 18:2/18:2
PC 38:4 5,507f 4,998 3,812 854 18:0 (283) 20:4 (303) LPC 18:0 (508) PC 18:0/20:4
PC 40:1 28f 42 18 888 16:0 (255) 24:1 (365) LPC 16:0 (480) PC 16:0/24:1
PC 42:1 36f 58 12 916 26:1 (393) 16:0 (255) LPC 26:1 (618) PC 26:1/16:0
24:0 (367) 18:1 (281) LPC 24:0 (592) PC 24:0/18:1
PC 42:2 29f 45 14 914 24:0 (367) 18:2 (279) LPC 24:0 (592) PC 24:0/18:2
24:1 (365) 18:1 (281) LPC 24:1 (590) PC 24:1/18:1
PC 42:3 21f 29 13 912 24:2 (363) 18:1 (281) LPC 24:2 (588) PC 24:2/18:1
PC 44:1 30f 45 1 944 26:0 (395) 18:1 (281) LPC 26:0 (620) PC 26:0/18:1
PC 44:2 23f 38 3 942 26:1 (393) 18:1 (281) LPC 26:1 (618) PC 26:1/18:1
PE 34:4 83 106 67 710 14:0 (227) 20:4 (303) LPE 14:1 (424) PE 14:0/20:4
SM 32:1 1,504 1,563h 1,135 719 16:0 (255) d16:1 (421) SM d16:1/16:0
14:0 (255) d18:1 (449) SM d18:1/14:0
SM 34:1 20,208h 13,219 11,769 747 16:0 (255) d18:1 (449) SM d18:1/16:0
SM 34:2 2,295 3,139f 2,099 745 16:0 (255) d18:2 (447) SM d18:2/16:0
SM 44:1 248f 193h 42 887 26:0 (395) d18:1 (449) SM d18:1/26:0
SM 44:2 421 356f 154 885 26:1 (393) d18:1 (449) SM d18:1/26:1
a

Each PL species observed by semi-quantitative analysis is represented by the total carbon and double bond number of two fatty acyl moieties (PC) or by the long-chain base and an N-acyl moiety (SM).

b

PC 38:0 and SM 44:3 could not be assigned because of the absence of a spectrum corresponding to 1-acyl LPC and N-acyl moiety in LC-MS3 analysis, respectively.

c

The quantity of each PL species in the CCALD, AMN, and control fibroblasts picomoles per milligram of protein).

d

The m/z of the first precursor ion was used to analyze the structure of each PL species in MS3 using the negative ion mode.

e

Notation in bold indicates fatty acyl moieties with no less than 24 carbons. FA, LPC, and SPC represent fatty acyl-moiety, LPC, and sphingosylphosphorylcholine (SPC), respectively.

f

P < 0.05 versus control (Dunnett T3 post hoc test).

g

P < 0.05 versus AMN (Dunnett T3 post hoc test).

h

P < 0.01 versus control (Dunnett T3 post hoc test).