TABLE 5.
Structure of the PL species present in quantities significantly higher in the ABCD1-KO HeLa cells
| Amountb | |||||||
| Signalsa | WT (n = 3) | KO (n = 3) | (m/z)c | sn-1 FA (m/z)d | sn-2 FA (m/z)d | 1-Acyl LPC (m/z)d | Molecular Species |
| PC 44:0 | n.q. | 3 | 946 | 28:0 (423) | 16:0 (255) | LPC 28:0 (648) | PC 28:0/16:0 |
| 26:0 (395) | 18:0 (283) | LPC 26:0 (620) | PC 26:0/18:0 | ||||
| PC 46:4 | n.q. | 2 | 966 | 26:0 (395) | 20:4 (303) | LPC 26:0 (620) | PC 26:0/20:4 |
a
Each PL species observed in semi-quantitative analysis is represented by the total carbon and double bond number of two fatty acyl moieties (PC).
b
The quantity of each PL species in the three WT and three ABCD1-KO (KO) HeLa cell lines (picomoles per milligram of protein). n.q., below the quantitation range.
c
The m/z of the first precursor ion was used to analyze the structure of each PL species in MS3 using the negative ion mode.
d
Notation in bold indicates fatty acyl moieties with no less than 24 carbons.