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TBX5 activates transcription from the ANF promoter. HEK293T cells were cotransfected with the ANF reporter plasmid and plasmids overexpressing TBX5, TBX5P85T, TBX5R264K. The TBX5R264K mutant activated the ANF promoter in a manner similar to the wild-type TBX5. Relative luminescence activity (mean ± SEM) is plotted.
