Table 3. Intrastrain heterogeneity found in the TEN Iraq B genome.
| TEN Iraq B (CP032303.1) coordinates | Reference allele | Alternative allele | Amino acid replacement | Average depth coverage (x) | Percentage of alternative allele (%) | Gene | Protein |
|---|---|---|---|---|---|---|---|
| 14661 | C | T | R/C | 1841 | 19.0 | pheT (TP0015) | phenylalanine—tRNA ligase, beta subunit |
| 135229 | G | T | P/E | 4380 | 44.5 | tprC (TP0117) | TprC |
| 135228 | G | C | P/E | 4360 | 44.4 | tprC | |
| 135246 | C | T | R/K | 3573 | 34.7 | tprC | |
| 136528 | C | T | E/K | 4264 | 26.0 | tprC | |
| 136542 | C | T | R/H | 3961 | 24.8 | tprC | |
| 334466 | G | A | A/T | 6111 | 8.1 | tmpC (TP0319) | sugar ABC superfamily ATP binding cassette transporter |
| 384160 | G | T | A/S | 3050 | 10.0 | cheA (TP0363) | sensor histidine kinase |
| 388371 | G | A | D/N | 2222 | 12.2 | cheY (TP0366) | response regulator |
| 824846 | G | A | A/A | 3614 | 12.7 | (TP0762) | hypothetical protein |
| 883419 | G | T | G/W, P/H | 2479 | 9.2 | (TP0814a, TP0814b) | hypothetical proteins |
| G/V | (TP0815) | GNAT family acetyltransferase | |||||
| 1091347 | G | C | A/P | 1005 | 9.3 | (TP1003) | hypothetical protein |
Minor alleles with a frequency over 8% and depth coverage over 100x are shown. The tprK (TP0897) gene was excluded from the analysis.