Skip to main content
. 2020 Apr 1;15(4):e0230044. doi: 10.1371/journal.pone.0230044

Fig 3. Effect of TGF-β on SGLT, GYS1 and on pGYS1 (Ser641) protein levels.

Fig 3

A. Original western blots of SGLT1, GYS1, pGYS1 (Ser641), and GAPDH protein in whole cell lysates from Ishikawa cells without or with a 48 hours treatment with LEFTY2 (25 ng/ml) or with TGF-β (10 ng/ml) or in combination. GAPDH was used as a loading control. B. Arithmetic means ± SEM (n = 10) of SGLT1/GAPDH, GYS1/GAPDH (n = 9) and pGYS1 (Ser641)/GAPDH protein (n = 6) abundance ratios in cell lysates. C. Original western blots of SGLT1, GYS1, pGYS1 (Ser641), and GAPDH protein in whole cell lysates from HEC1a cells without or with a 48 hours treatment with LEFTY2 (25 ng/ml) or with TGF-β (ng/ml) or together. GAPDH was used as a loading control. D. Arithmetic means ± SEM (n = 10) of SGLT1/GAPDH, GYS1/GAPDH (n = 5) and pGYS1 (Ser641)/GAPDH protein (n = 4) abundance ratios in cell lysates. *(p<0.05), **(p<0.01) indicates statistically significant difference from untreated cells.