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. 2020 Apr 1;15(4):e0231386. doi: 10.1371/journal.pone.0231386

Fig 7. ZEB2 downregulation induces apoptosis by the activation of Caspase-3 in glioma cells.

Fig 7

A. Apoptosis in U251 and U87 cells was measured by Annexin V-FITC/propidium iodide (PI) staining following siZEB2 or control treatment. Early apoptotic cell populations were significantly increased (P<0.01) after siZEB2 transfection. B. Western blot analysis for antiapoptotic PARP and effector Caspase-3, Caspase-6 and Caspase-9. Decreased PARP and increased caspase-3,-6,-9, and Cleaved PARP expression were observed in U251 and U87 cells after ZEB2 downregulation at 72 hr post transfection. β-actin was used as a loading control. Representative β-actin loading controls are shown for PARP and cleaved PARP; corresponding β-actin loading control panels are not available for Caspase-3, Caspase-6, and Caspase-9. Bar graph shows the relative expression of protein among the groups. Data are presented were presented as mean ± SD for three independent experiments. *P<0.05, statistically significant difference.