Fig 5. Melanopsin—β2-adrenergic receptor and melanopsin—Angiotensin II type-1a receptor chimeric mutants display different deactivation kinetics.

In both chimeric constructs, melanopsin was truncated at residue 387, which is right before P-II. (A) 2D schematic of melanopsin-β₂-adrenergic receptor chimera, melanopsin residues are represented in blue and β₂AR residues are represented in red. Phosphorylation sites on β₂AR are represented by diamond shaped residues. (B) 2D schematic of melanopsin-angiotensin II type-1a receptor chimera, melanopsin residues are represented in blue and ATII1aR residues are represented in green. Phosphorylation sites are represented by diamond shaped residues. (C & D) Zoomed in schematics of chimeric C-termini of melanopsin-β₂-adrenergic receptor chimera and melanopsin-angiotensin II type-1a receptor chimera. (E) Calcium imaging of chimeric mutants, error bars represent the S.D. of the transfection.