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. 2020 Apr 1;15(4):e0230423. doi: 10.1371/journal.pone.0230423

Table 1. Antimicrobial and resistance modulatory activity of (-)-α-pinene with antibiotics ciprofloxacin and erythromycin in 57 Campylobacter jejuni strains from chicken meat (strain code, CB), turkey meat (strain code, CT), human feces (strain codes F, X) and the reference strain NCTC 11168.

Strain (-)-α-Pinene Ciprofloxacin Erythromycin
code MIC (mg/L) MIC (mg/L) Fold MIC (mg/L) Fold
Alone Alone Plus (-)-α-pinenea changeb Alone Plus (-)-α-pinenea changeb
CB1:6 1000 16 <0.125 >128 0.5 <0.002 >256
CB1:14 1000 16 <0.125 >128 0.5 <0.002 >256
CB1:18 1000 16 <0.125 >128 0.5 0.125 4
CB2:6 2000 64 16 4 0.5 0.06 8
CB2:8 2000 64 32 2 0.5 0.25 2
CB2:11 1000 8 4 2 0.5 0.25 2
CB3:1 2000 0.125 0.06 2 0.25 0.06 4
CB3:5 2000 0.25 0.06 4 0.5 0.25 2
CB 4:21 1000 0.06 0.03 2 0.06 <0.002 >32
CB 4:22 2000 0.125 0.001 128 0.06 0.002 32
CB 6:8 2000 0.06 0.008 8 0.125 <0.002 >64
CB 6:9 2000 0.06 0.03 2 0.125 0.06 2
CB 6:26 2000 0.06 0.03 2 0.25 0.125 2
CB 7:15 1000 8 <0.06 >128 0.25 <0.002 >128
CB 7:21 2000 8 1 8 0.125 <0.002 >64
CB 8:14 2000 0.06 0.001 64 0.125 0.002 64
CB 8:15 1000 0.06 0.001 64 0.5 <0.002 >256
CT 1:1 2000 16 1 16 0.5 0.03 16
CT 1:9 1000 16 2 8 0.5 0.06 8
CT 2:2 2000 16 <0.06 >256 256 <1 >256
CT 3:5 2000 0.06 <0.001 >64 0.03 <0.002 16
CT3:11 500 4 <0.06 >64 - - -
CT3:19 2000 16 <0.06 >256 512 <1 >512
CT4:4 2000 16 8 2 128 64 2
CT4:14 2000 8 8 1 128 64 2
CT5:2 2000 16 4 4 256 256 1
CT5:8 2000 8 2 4 256 16 16
CT5:10 2000 16 <0.06 >256 256 <1 >256
CT5:12 2000 16 4 4 256 32 8
CT5:18 2000 16 4 4 256 64 4
CT 6:18 2000 8 0.5 16 128 <1 >128
CT 6:8 2000 16 2 8 256 <1 >256
CT 6:16 2000 0.03 <0.001 >32 128 <1 >128
CT 7:2 2000 0.03 <0.001 >32 0.06 0.002 32
CT 8: 28 2000 8 1 8 0.25 <0.008 >32
CT 8:29 >2000 4 <0.06 >64 64 8 8
CT 8:22 2000 4 0.5 8 0.5 0.5 1
CT 9:14 2000 0.25 <0.002 >128 2 <0.008 >256
CT 10:18 1000 0.06 <0.002 >32 0.25 <0.008 >32
CT 9:21 1000 0.125 <0.002 >64 2 <0.008 >256
F6501 >2000 0.125 0.06 2 0.25 0.125 2
H2958 2000 0.125 0.03 4 0.5 0.25 2
M63885 2000 0.25 <0.002 >128 0.5 <0.008 >64
T59822 2000 0.125 0.016 8 0.25 0.06 4
W14861 >2000 0.25 0.25 1 2 2 1
X60179 2000 8 <0.06 >128 0.25 <0.002 >128
F15871 >2000 0.125 0.06 2 1 0.5 2
W11805 2000 0.06 0.031 2 0.25 0.06 4
M402 >2000 0.06 0.031 2 1 0.5 2
W28752 2000 0.06 0.008 8 0.5 <0.002 >256
M33323 2000 0.06 0.06 1 0.25 0.25 1
W64861 2000 0.125 0.06 2 0.25 0.25 1
M76297 2000 0.06 0.03 2 0.25 0.125 2
E46972 >2000 1 <0.002 >512 0.25 <0.008 >32
M36292 2000 0.06 <0.002 >32 0.25 <0.008 >32
X7199 >2000 16 16 1 0.25 0.125 >32
NCTC 11168 2000 0.06 0.03 2 0.25 0.06 4
MIC90c 2000 16 4 4 256 16 16
MIC50d 2000 0.25 0.06 4 0.5 0.06 8

a Addition of (subinhibitory) 125 mg/L (-)-α-pinene

b Fold change (improvement) of MIC with addition of 125 mg/L (-)-α-pinene

c Concentration that inhibits 90% of the tested strains [24]

d Concentration that inhibits 50% of the tested strains [24]