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Model for discontinuous negative‐strand transcription. Negative‐strand sgRNAs are initiated at the 3′ end of the gRNA template. Elongation proceeds as far as a body copy of a transcription‐regulating sequence (TRS). A strand‐switching event then occurs, pairing the newly transcribed negative‐sense body TRS with the leader copy of the TRS, from which point transcription resumes. A complex of the (+)gRNA and the (−)sgRNA then serves as the template for synthesis of multiple (+)sgRNAs.
