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. 2006 Sep 22;68:287–320. doi: 10.1016/S0065-3527(06)68008-1

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Copyright © 2006 Elsevier Inc. All rights reserved.

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Fig 2 — Baculovirus transduction of adipogenic, osteogenic, and chondrogenic progenitors. Human mesenchymal stem cells (MSC) were induced to differentiate into adipogenic, osteogenic, and chondrogenic pathways. The adipogenic, osteogenic, and chondrogenic progenitors were revealed by staining with oil red‐O, von Kossa, and safranin‐O staining, respectively, two weeks postinduction (left panels of each lineage pathway). The progenitors were transduced with baculoviruses expressing enhanced green fluorescent protein (EGFP) 2 weeks postinduction and exhibited different degrees of EGFP expression (right panel of each lineage pathway). The transduction efficiency (TE) and mean fluorescence intensity (MFI) were measured by flow cytometry.