Fig 1.

Virus production at the apical (A) and basal (B) sides of air-liquid interface culture of reconstituted human airway epithelia. Viral RNA loads measured by using qPCR from infected samples collected at the apical (Fig 1, A) or basal (Fig 1, B) side of tissues infected with 9 different respiratory viruses (n ≥ 4) are shown. Each tissue was inoculated apically with 10⁶ RNA copies of the indicated virus and washed 3 times after 4 hours. Samples were then collected from the apical or basal face at the indicated time point. Input, Viral RNA load present in the inoculum for each viral stock; p.i., post-inoculation; Residual, residual bound virus after 3 washes.