Fig 2.

Immunofluorescence of epithelia infected with the indicated respiratory virus. A, Three-dimensional view of noninfected and infected tissues, with ciliated cells stained in green, viruses in red, and cell nuclei in blue. For all viruses, immunofluorescence was performed at day 5 after infection, whereas for H3N2 and EV-D68, immunofluorescence was also done at day 2, as indicated. B, Two-dimensional view of tissues infected with H3N2 at day 5 after infection (virus stained in green, mucus cells in red [Muc5A], basal cells in red [P63], and cell nuclei in blue), with magnification insets of selected regions (*) in each panel. DAPI, 4′-6-Diamidino-2-phenylindole dihydrochloride.