Table 2.
Summary of Rate Constants of Elemental Translation Steps upon –1PRF on the IBV 1a/1b Construct
| SS/PK | Rates (s−1) | |||||
|---|---|---|---|---|---|---|
| Tyra | TlMYb | Leua | Lysa | Phea | Vala | |
| 0 frame | –1 frame | |||||
| +/+ | 10.1 ± 0.5 | 1.9 ± 0.1 | 11.1 ± 3.5 | 4.3 ± 0.5 | 0.09 ± 0.01 | 0.3 ± 0.05 |
| +/– | 9.8 ± 0.4 | 1.4 ± 0.1 | 14.9 ± 6.3 | 6.0 ± 0.9 | 5.9 ± 1.1 | 2.4 ± 0.5 |
| –/+ | 8.8 ± 0.4 | 3.1 ± 0.2 | 17.8 ± 5.6 | 4.2 ± 0.4 | 0.04 ± 0.02 | 0.06 ± 0.02 |
| –/– | 9.9 ± 0.5 | 2.7 ± 0.1 | 13.6 ± 3.2 | 5.1 ± 0.4 | 5.3 ± 0.6 | 0.2 ± 0.05 |
| 0 frame | ||||||
| –/– | 11.1 ± 0.8 | 2.2 ± 0.1 | 14.4 ± 6 | 6.3 ± 1.2 | NA | 4.0 ± 0.4 |
See also Figures S1 and S3 and Table S1.
a
Rates of incorporation of the respective amino acid into peptide, as determined by global fitting of data shown in Figures 1D–1H.
b
Rates of the first translocation event (TlMY; translocation of MY-tRNATyr), which is manifested by a delay of Leu incorporation (Figures 1D–1H).