Table 1.
Overview of Ub and Ubl ABPs used in this study
| Protein | Abbreviation | UniProt ID | Residuesa | MW (kDa) |
|---|---|---|---|---|
| Ubiquitin | Ub | P0CG47b | 1-75 | 8.5 |
| Nedd8 | N8 | Q15843 | 1-75 | 8.5 |
| SUMO1 | S1 | P63165 | 1-96 | 11.1 |
| SUMO2 | S2 | P61956 | 1-92 | 10.6 |
| SUMO3 | S3 | P55854 | 1-91 | 10.5 |
| ISG15 C-domain | I15c | P05161 | 79–156 | 8.9 |
| ISG15 (C78S)c | I15 | P05161 | 1–156 | 17.1 |
a
The C-terminal Gly residue is not included in this list.
b
Ub is only listed as a polyubiquitin in UniProt; the UniProt ID refers to polyubiquitin-B (UBB).
c
The C78S-mutation was introduced to solubilize the ISG15-protein.