Fig. 6.

Lysosomal localization of cystatin F in adherent dendritic cells (DCs): Part of image is magnified in lower right corner. The white colour indicates colocalization of two labelled antigens, confirming the presence of cystatin F in lysosomes. The threshold value for colocalization was set to one half of the maximal brightness level. The mask of the pixels above the threshold in both channels (significant colocalization, blue colour) and the contour plot are shown (Magister et al., 2012). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)