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. 2006 Jun 2;39(1):151–156. doi: 10.1016/j.ijbiomac.2006.05.010

Fig. 2.

Fig. 2

Strep-tag affinity chromatography. (A) Purificaton yield. Percentage of IC1-stII protein in cellular supernatants before (black bar) and after (white bar) loading into the strep-tactin column, both for non-diluted (1) and 1:10 diluted (2) samples. The OD at 490 nm resulting from ELISA assays were normalized to that of non-diluted initial cell supernatant. (B) Efficiency of protein elution. OD at 490 nm from an IC1 ELISA assay of the indicated elution fractions from a strep-tactin column (see Section 2). Protein samples were diluted 1:2 prior to the ELISA test. (C) Purity of column elution fractions containing IC1-stII. A 10% SDS-PAGE of cellular supernatant before (b) and after (a) loading onto the strep-tactin column and of the indicated elutions fractions included in panel B. Arrow marks the approximate migration of the IC1-stII protein. Size and migration of molecular weight markers are indicated.