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. 2006 Jun 2;39(1):151–156. doi: 10.1016/j.ijbiomac.2006.05.010

Fig. 3.

Fig. 3

Immunoaffinity chromatography. (A) Purificaton yield. Percentage of IC1-His protein in the cellular supernatant before (black bar) and after (white bar) loading into a column having an anti-IC1 antibody coupled to sepharose (see Section 2), both for non-diluted (1) and 1:10 diluted (2) samples. (B) Efficiency of protein elution from the anti-IC1 R6.5 antibody column determined essentially as in the previous figures. Protein samples were diluted 1:100 prior to the ELISA test. (C) Protein content of antibody column elution fractions determined by 10% SDS-PAGE as in Fig. 2.