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. 2006 Jun 2;39(1):151–156. doi: 10.1016/j.ijbiomac.2006.05.010

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Copyright © 2006 Elsevier B.V. All rights reserved.

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Fig. 4 — Purification of the CoVSH1 glycoprotein. (A) Protein content of fractions recovered from low pH elution of an anti-flag M2-antibody column (see Section 2). A 10% SDS-PAGE of the indicated fraction number, with the arrow pointing to the CoVSH1 protein. Size and migration of molecular weight markers are indicated. (B) SDS-PAGE of treated (right) and untreated (left) CoVSH1 glycoprotein. (C) SDS-PAGE of the indicated fraction number recovered from an ion exchange mono Q column (see Section 2).