Fig. 4.

Granzyme (a) and IFN-γ (b) production activity in NK cells isolated from CBP1S-stimulated Balb/c splenocytes. NK cells (effector cells) collected from CBP1S-injected Balb/c mice were co-cultured with Yac-1 lymphoma cells (target cells) with different effector to target ratios (25:1, 50:1, and 100:1). The production of NK cell-activating substances (granzyme and IFN-γ) was determined in the culture supernatant by sandwich ELISA. The negative control (NC) was NK cells isolated from saline-injected Balb/c mice. Means with different superscript letters indicate significant differences at p < 0.05 by Duncan's multiple range test.