Figure 2.

Nucleocapsid-specific T-cell responses (upper) and neutralising capacity (lower) of serum samples after vaccination

Immunised animals represented by black symbols, controls by white symbols. (Upper) ELISPOT analysis 24 h after monkey peripheral blood mononuclear cells (PBMC) were incubated with pools of nucleocapsid-specific peptides and interferon-γ (IFN-γ) spot-forming cells (SFC) were counted. Data are mean (SE) of triplicate determinations after subtraction of background responses. (Lower) Detection of virus-specific neutralising antibodies in serum by inhibition of E6 Vero cell lysis by SARS-CoV strain Urbani in a microneutralisation assay. Data for 1699 and 1201 overlap at all time points.