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. 2019 Nov 13;117:37–53. doi: 10.1016/j.molimm.2019.10.017

Fig. 2.

Fig. 2

Western blotting of deiminated proteins and PAD in llama serum. A. Llama PAD homologues were identified at the expected size of approximately 70–75 kDa using the anti-human PAD2, PAD3 and PAD4 isozyme specific antibodies respectively. Deiminated histone H3 (citH3), representative of neutrophil extracellular traps (NETs), was verified in llama serum. B. Total deiminated proteins were assessed by Western blotting in llama serum EVs, using the F95 pan-deimination specific antibody. C. Immunoprecipitated (IP) deiminated proteins after F95-enrichment were assessed both in serum-EVs and whole serum of llama by Western blotting. D. The Venn diagram represents deiminated proteins identified in total serum and serum-derived EVs by F95 enrichment and LC–MS/MS analysis. Overall, 43 proteins were identified in common with both samples, while 60 proteins were found deiminated in serum only and 25 proteins were identified as deiminated in EVs only.