Table 1.
Deiminated proteins identified by F95 enrichment and LC–MS/MS in total serum of llama (Lama glama). Deiminated proteins were isolated by immunoprecipitation using the pan-deimination F95 antibody. The F95 enriched eluate was analysed by LC–MS/MS and peak list files were submitted to in-house Mascot (Cambridge Proteomics). Peptide sequence hits scoring with Lama glama (LAMGL) are included as well as hits with other camelids (CAMFR=Camelus ferus; CAMDR=Camelus dromedaries; LAMGU=Lama guanicoe; VICPA=Vicugna pacos (Alpaca)). Hits with uncharacterized proteins are omitted in the list. For a full list of peptide sequences and m/z values see Supplementary Table 1. An asterix (*) indicates that the protein hit is specific to whole serum only.
| Protein name (*unique for serum) | Number of peptide sequences identified | Total score (p<0.05)ⱡ |
|---|---|---|
| *O97643_LAMGL | 6 | 282 |
| Fibrinogen A-alpha chain | ||
| *P01973|HBA_LAMGL | 5 | 189 |
| Hemoglobin subunit alpha | ||
| P68226|HBB_LAMGL | 4 | 166 |
| Hemoglobin subunit beta | ||
| Q865W8_LAMGL | 4 | 109 |
| Beta actin | ||
| A0A1W5VKM5_LAMGL | 2 | 117 |
| Anti-RON nanobody | ||
| *A0A1W5VKM7_LAMGL | 2 | 111 |
| Anti-RON nanobody | ||
| *A0A1W5VKR8_LAMGL | 2 | 96 |
| Anti-RON nanobody | ||
| *A0A1W5VKQ9_LAMGL | 1 | 66 |
| Anti-RON nanobody | ||
| S9XDK9_CAMFR | 34 | 1575 |
| Complement C3-like protein | ||
| S9WI87_CAMFR | 20 | 972 |
| Serum albumin | ||
| S9XAP9_CAMFR | 9 | 522 |
| Keratin, type I cytoskeletal 14-like protein | ||
| S9Y6J1_CAMFR | 11 | 502 |
| Keratin, type II cytoskeletal 5 isoform 13-like protein | ||
| *S9 × 688_CAMFR | 10 | 462 |
| Keratin 6A-like protein | ||
| *S9YD43_CAMFR | 9 | 443 |
| Complement component 4A-like protein | ||
| *S9Y253_CAMFR | 8 | 380 |
| Kininogen-2 isoform I | ||
| *T0MII3_CAMFR | 7 | 371 |
| Alpha-2-macroglobulin-like protein | ||
| S9XI90_CAMFR | 8 | 357 |
| Keratin, type II cytoskeletal 75-like isoform | ||
| S9 × 494_CAMFR | 6 | 285 |
| Keratin, type I cytoskeletal 42 | ||
| S9XBS9_CAMFR | 6 | 263 |
| Ig gamma-3 chain C region | ||
| *S9XYY2_CAMFR | 5 | 246 |
| Hemopexin | ||
| A0A075T9L1_CAMDR | 4 | 174 |
| Dipeptidylpeptidase 4 | ||
| S9XXW2_CAMFR | 3 | 172 |
| Fibrinogen beta chain | ||
| *S9WDV3_CAMFR | 3 | 169 |
| Fibrinogen gamma chain isoform gamma-B | ||
| *A0A1K0GY87_VICPA | 4 | 166 |
| Globin A1 | ||
| S9WB99_CAMFR | 3 | 146 |
| Histone H2B | ||
| S9XNF8_CAMFR | 3 | 142 |
| Xaa-Pro dipeptidase | ||
| *S9YS49_CAMFR | 3 | 132 |
| Putative E3 ubiquitin-protein ligase Roquin | ||
| *S9YGW7_CAMFR | 3 | 123 |
| Heparin cofactor 2 | ||
| S9WPM4_CAMFR | 3 | 115 |
| Adiponectin | ||
| A2V743_CAMDR | 4 | 109 |
| Beta actin | ||
| *S9XP08_CAMFR | 1 | 109 |
| Inter-alpha-trypsin inhibitor heavy chain H1 | 2 | |
| S9XM15_CAMFR | 2 | |
| Ferritin | ||
| T0NNK2_CAMFR | 1 | 93 |
| L-lactate dehydrogenase | ||
| S9 × 3E8_CAMFR | 1 | 90 |
| Ig kappa chain V-II region RPMI 6410-like protein | ||
| A0A0A0PAR2_CAMDR | 2 | 88 |
| Heat shock protein 90 | ||
| *S9XYF2_CAMFR | 2 | 87 |
| Heat shock cognate protein HSP 90-beta-like isoform 3 | ||
| S9XHZ4_CAMFR | 1 | 83 |
| Phosphotriesterase-related protein | ||
| S9XM68_CAMFR | 2 | 73 |
| Xaa-Pro dipeptidase isoform 3 | ||
| S9WT57_CAMFR | 2 | 73 |
| Tubulin beta chain | ||
| *S9YV02_CAMFR | 1 | 69 |
| Non-specific protein-tyrosine kinase | ||
| *S9XC57_CAMFR | 1 | 63 |
| Plasminogen | ||
| *S9YL21_CAMFR | 2 | 62 |
| Apolipoprotein A-I | ||
| *S9WKZ8_CAMFR | 1 | 62 |
| Inter-alpha-trypsin inhibitor heavy chain H4 | ||
| S9WIA5_CAMFR | 1 | 61 |
| Glutathione synthetase | ||
| T0MHN9_CAMFR | 1 | 60 |
| Pyruvate kinase | ||
| *S9Y4U4_CAMFR | 1 | 58 |
| Complement C1q subcomponent subunit C isoform 2 | ||
| S9WAX5_CAMFR | 2 | 57 |
| Unconventional myosin-Va isoform 2 | ||
| S9WVY1_CAMFR | 1 | 56 |
| Actinin, alpha 1 isoform 6-like protein | ||
| S9XA40_CAMFR | 1 | 55 |
| Heat shock cognate protein | ||
| *S9YFM0_CAMFR | 1 | 52 |
| Keratin, type II cytoskeletal 71 | ||
| A0A0U2KTX5_CAMDR | 1 | 52 |
| VHH5 (Fragment) | ||
| S9XR87_CAMFR | 1 | 50 |
| Ig lambda chain C regions isoform 19-like protein | ||
| S9WGH8_CAMFR | 1 | 49 |
| Lysozyme | ||
| S9WMX2_CAMFR | 2 | 49 |
| Dystonin | ||
| *S9 × 8K9_CAMFR | 1 | 46 |
| Transaldolase | ||
| *T0NM23_CAMFR | 2 | 45 |
| Rootletin | ||
| S9W6I0_CAMFR | 1 | 45 |
| Ferritin | ||
| S9WF34_CAMFR | 1 | 43 |
| Tubulin alpha chain | ||
| *S9YSI7_CAMFR | 1 | 43 |
| Triosephosphate isomerase | ||
| *S9XSQ6_CAMFR | 1 | 40 |
| Vitamin d-binding protein-like protein | ||
| *S9YMC0_CAMFR | 2 | 40 |
| Transcription factor 20 isoform 1 | ||
| *S9Y636_CAMFR | 1 | 39 |
| Receptor-type tyrosine-protein phosphatase-like N | ||
| *S9 × 6M4_CAMFR | 1 | 37 |
| Dyslexia-associated protein | ||
| T0MH94_CAMFR | 1 | 36 |
| Rabenosyn-5-like protein | ||
| S9WJW3_CAMFR | 1 | 35 |
| N6-adenosine-methyltransferase subunit | ||
| *A8IY99_LAMGU | 1 | 35 |
| Gamma-fibrinogen | ||
| S9WRI7_CAMFR | 1 | 35 |
| Nuclear receptor coactivator 5 isoform 3-like protein | ||
| S9W421_CAMFR | 1 | 35 |
| Hemoglobin, epsilon 1 | ||
| *S9W711_CAMFR | 1 | 34 |
| Charged multivesicular body protein 4c | ||
| *S9 × 089_CAMFR | 1 | 34 |
| Ig lambda chain V-III region LOI-like protein | ||
| S9WUC8_CAMFR | 1 | 32 |
| Ig kappa chain V-II region RPMI 6410-like protein | ||
| *S9WVI6_CAMFR | 1 | 32 |
| Complement C1q subcomponent subunit A | ||
| *S9Y5S1_CAMFR | 1 | 32 |
| Transcriptional repressor NF-X1 | ||
| *S9YC53_CAMFR | 1 | 32 |
| Alpha-1-antitrypsin-like protein | ||
| *S9Y3F6_CAMFR | 1 | 31 |
| Dual specificity testis-specific protein kinase 1 | ||
| S9WKI8_CAMFR | 1 | 31 |
| HEAT repeat-containing protein 7B1 | ||
| *S9WVS9_CAMFR | 1 | 29 |
| Peroxisome proliferator-activated receptor gamma coactivator-related protein 1 | ||
| *S9XVK5_CAMFR | 1 | 29 |
| Transthyretin | ||
| *S9Y967_CAMFR | 1 | 28 |
| General transcription factor II, i isoform 4 isoform 1-like protein | ||
| *T0MC04_CAMFR | 1 | 28 |
| Spermatogenesis-associated protein 2-like protein | ||
| *S9YSZ6_CAMFR | 1 | 28 |
| Centromere protein J |
Ions score is -10*Log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicated identity or extensive homology (p < 0.05). Protein scores were derived from ions scores as a non-probabilistic basis for ranking protein hits. Cut-off was set at Ions score 20.