Table 2.
Deiminated proteins identified by F95 enrichment and LC–MS/MS in EVs isolated from serum of llama (Lama glama). Deiminated proteins were isolated by immunoprecipitation using the pan-deimination F95 antibody, the F95 enriched eluate was analysed by LC–MS/MS and peak list files were submitted to Mascot. Peptide sequence hits scoring with L. Lama glama (LAMGL) are presented as well as hits with other camelids (CAMFR=Camelus ferus; CAMDR=Camelus dromedaries; LAMGU=Lama guanicoe). Hits with uncharacterised proteins are not listed. For a full list of peptide sequences and m/z values see Supplementary Table 2. An asterix (*) indicates that the protein hit is unique for EVs only.
| Protein name (*unique for EVs) | Number of peptide sequences identified | Total score (p<0.05)ⱡ |
|---|---|---|
| A0A1W5VKM5_LAMGL | 2 | 164 |
| Anti-RON nanobody | ||
| Q865W8_LAMGL | 2 | 85 |
| Beta actin | ||
| *S9XAP9_CAMFR | 9 | 554 |
| Keratin, type I cytoskeletal 14-like protein | ||
| *S9 × 688_CAMFR | 10 | 496 |
| Keratin 6A-like protein | ||
| S9Y6J1_CAMFR | 10 | 438 |
| Keratin, type II cytoskeletal 5 isoform 13-like protein | ||
| S9WI87_CAMFR | 9 | 430 |
| Serum albumin | ||
| *S9YN99_CAMFR | 6 | 417 |
| Keratin, type I cytoskeletal 17-like isoform | ||
| *S9XI90_CAMFR | 6 | 318 |
| Keratin, type II cytoskeletal 75-like isoform | ||
| S9 × 494_CAMFR | 5 | 269 |
| Keratin, type I cytoskeletal 42 | ||
| S9XBS9_CAMFR | 4 | 162 |
| Ig gamma-3 chain C region | ||
| A0A075T9L1_CAMDR | 3 | 153 |
| Dipeptidylpeptidase 4 | ||
| S9 × 684_CAMFR | 2 | 136 |
| Keratin, type II cytoskeletal 8 | ||
| S9WB99_CAMFR | 3 | 133 |
| Histone H2B | ||
| S9YQ51_CAMFR | 3 | 114 |
| Tubulin beta chain | ||
| *S9WX81_CAMFR | 3 | 89 |
| Histone 1, H2ai isoform 3-like protein | ||
| *S9 × 8G9_CAMFR | 2 | 88 |
| Desmoplakin | ||
| A2V743_CAMDR | 2 | 85 |
| Beta actin | ||
| A0A0A0PAR2_CAMDR | 2 | 85 |
| Heat shock protein 90 | ||
| S9XA40_CAMFR | 1 | 85 |
| Heat shock cognate protein | ||
| T0NNK2_CAMFR | 1 | 81 |
| L-lactate dehydrogenase | ||
| S9XNF8_CAMFR | 2 | 74 |
| Xaa-Pro dipeptidase | ||
| T0MHN9_CAMFR | 1 | 71 |
| Pyruvate kinase | ||
| S9WVY1_CAMFR | 1 | 60 |
| Actinin, alpha 1 isoform 6-like protein | ||
| *A0A0E3Z5I3_CAMDR | 1 | 59 |
| Superoxide dismutase | ||
| S9XHZ4_CAMFR | 1 | 57 |
| Phosphotriesterase-related protein | ||
| S9W9Y4_CAMFR | 1 | 57 |
| Ferritin | ||
| S9XR87_CAMFR | 1 | 56 |
| Ig lambda chain C regions isoform 19-like protein | ||
| S9 × 3E8_CAMFR | 1 | 50 |
| Ig kappa chain V-II region RPMI 6410-like protein | ||
| S9WAX5_CAMFR | 2 | 49 |
| Unconventional myosin-Va isoform 2 | ||
| S9WF34_CAMFR | 1 | 44 |
| Tubulin alpha chain | ||
| *S9W806_CAMFR | 2 | 43 |
| Filamin-A isoform 1 | ||
| *S9 × 6X3_CAMFR | 2 | 42 |
| Scaffold attachment factor B-like protein | ||
| T0MH94_CAMFR | 1 | 40 |
| Rabenosyn-5-like protein | ||
| *S9Y0S0_CAMFR | 1 | 39 |
| DNA-directed RNA polymerase subunit beta | ||
| S9WJW3_CAMFR | 1 | 38 |
| N6-adenosine-methyltransferase subunit | ||
| S9XM68_CAMFR | 1 | 38 |
| Xaa-Pro dipeptidase isoform 3 | ||
| *S9YV02_CAMFR | 1 | 38 |
| Non-specific protein-tyrosine kinase | ||
| S9WGH8_CAMFR | 1 | 32 |
| Lysozyme | ||
| S9WRI7_CAMFR | 1 | 32 |
| Nuclear receptor coactivator 5 isoform 3-like protein | ||
| S9W421_CAMFR | 1 | 32 |
| Hemoglobin, epsilon 1 | ||
| *S9WI71_CAMFR | 1 | 32 |
| Metabotropic glutamate receptor 3 | ||
| *S9WB50_CAMFR | 1 | 31 |
| TSC22 domain family protein 3-like protein | ||
| S9WMX2_CAMFR | 1 | 31 |
| Dystonin | ||
| S9WKI8_CAMFR | 1 | 31 |
| HEAT repeat-containing protein 7B1 | ||
| S9WIA5_CAMFR | 1 | 31 |
| Glutathione synthetase | ||
| *S9XC05_CAMFR | 1 | 31 |
| Telomere-associated protein RIF1 isoform 1 | ||
| A0A0U2KTX5_CAMDR | 1 | 30 |
| VHH5 | ||
| *T0MGG7_CAMFR | 1 | 30 |
| Nucleoredoxin | ||
| *S9XET3_CAMFR | 1 | 30 |
| Rac GTPase-activating protein 1 | ||
| S9XDK9_CAMFR | 1 | 30 |
| Complement C3-like protein | ||
| *S9XMI2_CAMFR | 1 | 29 |
| Pseudopodium-enriched atypical kinase 1 | ||
| *S9Y3S9_CAMFR | 1 | 29 |
| Core histone macro-H2A.1 isoform 2 | ||
| S9XXW2_CAMFR | 1 | 29 |
| Fibrinogen beta chain | ||
| *S9YGX6_CAMFR | 1 | 29 |
| PAS domain-containing serine/threonine-protein kinase | ||
| *T0NMU1_CAMFR | 1 | 28 |
| SH2 domain-containing protein 7 | ||
| *T0MIT6_CAMFR | 1 | 28 |
| Serine-tRNA ligase, mitochondrial | ||
| *S9W449_CAMFR | 1 | 28 |
| Fc receptor-like protein 5 |
Ions score is -10*Log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicated identity or extensive homology (p < 0.05). Protein scores were derived from ions scores as a non-probabilistic basis for ranking protein hits. Cut-off was set at Ions score 20.