Table 3.

Reaction mixture for real-time PCR for amplification of N gene of IBV.

Sr. No.	Reaction components	Volume
1	Diluted cDNA (1:10 in NFW)	2.0 μl
2	SYBR® Green master mix	10.0μl
3	Forward primers (N gene)	0.5 μl
4	Reverse primer (N gene)	0.5 μl
5	RNase-free water	7.0 μl
	Total Volume	20.0μl