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. 2020 Feb 14;120:52–60. doi: 10.1016/j.molimm.2020.02.001

Table 5.

Primers used for quantitative real time PCR (qPCR) analysis.

Gene Primer Sequence (5′-3′) References
IFN-α F: ATC CTG CTG CTC ACG CTC CTT CT
R: GGT GTT GCT GGT GTC CAG GAT G
Paul et al. (2011)
IFN-β F: ACA CTG ACA AGT CAA AGC CGC ACA
R: AGT CGT TCA TCG GGA GCT TGG C
Villanueva et al. (2011)
IFN-γ F: GCC TCC AGC TCC TTC AGA ATA CG
R: CTG GAT CTG GTT GAG GAG GCT GT
Brisbin et al. (2010)
OAS F: AGA ACT GCA GAA GAA CTT TGT C
R: GCT TCA ACA TCT CCT TGT ACC
Villanueva et al. (2011)
IL 1-β F:GTG AGG CTC AAC ATT GCG CTG TA
R: TGT CCA GGC GGT AGA AGA TGA AG
Paul et al. (2011)
iNOS F: GGC AGC AGC GTC TCT ATG ACT TG
R: GAC TTT AGG CTG CCC AGG TTG
Abdul-Careem et al., 2007
β-Actin F: CAA CAC AGT GCT GTC TGG TGG TA
R: ATC GTA CTC CTG CTT GCT GAT CC
Paul et al. (2011)
N gene F: GAAGAAAACCAGTCCCAGATGCTTGG
R: GTTGGAATAGTGCGCTTGCAATACCG
Dar et al.(2009)