. 2018 Aug 15;58:36–43. doi: 10.1016/j.cimid.2018.08.002

Table 1.

Oligonucleotide features used in the PCR for the detection of common enteric canine viruses.

Target	Primer	Sequence (5'-3')	Gene target	Product size (bp)	Reference
Astrovirus (pan-Astroviridae)	Astro F1	GARTTYGATTGGRCKCGKTAYGA	RdRp	422	Chu et al. [8]
	Astro F2	GGYTTKACCCACATNCCRAA
	Astro R	CGKTAYGATGGKACKATHCC
	Astro F3	AGGTAYGATGGKACKATHCC
	Astro F4	GARTTYGATTGGRCKAGGTAYGA
Mamastrovirus 5	46F	ATGTGTTCAGTGCCACCTTA	NSP1a	359	This study
Mamastrovirus 5	405R	CTTGTGTAAGCCTGTGCTGC	NSP1a	359	This study
Canine protoparvovirus 1	CPV 555F	CAGGAAGATATCCAGAAG	VP2	555	Buonavoglia et al. [51]
	CPV 555R	GGTGCTAGTTGATATGTA	VP2	555	Buonavoglia et al. [51]
Canine adenovirus 1 and 2	HA1	CGCGCTGAACATTACTACCTTGTC	E3	1307	Linné [52]
	HA2	CCTAGAGCACTTCGTGTCCGCTT	E3	1307	Linné [52]
Canine coronavirus	CCoV 1F	TCCAGATATGTAATGTTCGG	M	450	Herrewegh et al. [53]
	CCoV 2R	TCTGTTGAGTAATCACCAGCT	M	450	Herrewegh et al. [53]
Canine rotavirus	BEG 9F	GGCTTTAAAAGAGAGAATTTCCGTCTGG	VP7	1062	Gouvea et al. [54]
	END 9R	GGTCACATCATACAATTCTAATCTAAG	VP7	1062	Gouvea et al. [54]
Canine distemper virus	CDV 1F	ACTGCTCCTGATACTGC	NC	480	Castilho et al. [55]
	CDV 2R	TTCAACACCRACYCCC	NC	480	Castilho et al. [55]
	CDV 3F	ACAGRATTGCYGAGGACYTRT	NC	287	Frisk et al. [56]
	CDV 4R	CARRATAACCATGTAYGGTGC	NC	287	Frisk et al. [56]
Internal control	FC27	AGAGTTTGATCCTGGCTCAG	16S rRNA	530	Gontang et al. [17]
	R530	CCGCGGCTGCTGGCACGTA	16S rRNA	530	Gontang et al. [17]

R = A/G; Y = C/T; K = G/T; H = A/C/T; N = A/C/G/T. Bold characters indicate modifications introduced to the original sequences published in the references.