Fig. 5.

Detection of E2-specific antibodies using ELISA and western blot. (A) Baculovirus-expressed E2 protein was used as the ELISA antigen and serum (diluted 1:100) from rMV-E1E2-infected mice (10¹–10² pfu) was analysed. Anti-E2 monoclonal antibody (MoAb 544) was used as positive control. The asterisk (*) indicates a significant reaction (p < 0.05) compared to NMS. (B) An anti-E2 monoclonal antibody (MoAb 544), serum from rMV-E1E2 infected mice (1:100), or normal mouse serum (1:100) was used as primary antibodies in a western blot to detect baculovirus-expressed E2 protein. The triangles indicate bands that correspond to HCV E2.