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. 2019 Jul 24;114:100–107. doi: 10.1016/j.molimm.2019.07.003

Table 1.

Primers used for the construction of plasmids and qPCR assays.

Primer Nucleotide sequence (5–3)
nsp15-F GCGGAATTCAACCTTGAAAACTTAGCTTACAAC
nsp15-R ATACTCGAGCTGTAAGATTGGGTAGCAAGTCTT
H219A-F GGTGTTGAAGCCATTATCTATGGTGATGAT
H219A-R ATAGATAATGGCTTCAACACCCAGTTCCTG
H234A-F GGCGGAACTGCCACACTTATCTCACTAGTT
H234A-R GATAAGTGTGGCAGTTCCGCCAATGACTGG
K269A-F TAACGCAAGCTCCGCGAACGTTTGCACTGT
K269A-R GCAAACGTTCGCGGAGCTTGCGTTAGGTGA
h-IFN-β-F TCTTTCCATGAGCTACAACTTGCT
h-IFN-β-R GCAGTATTCAAGCCTCCCATTC
h-GAPDH-F TCATGACCACAGTCCATGCC
h-GAPDH-R GGATGACCTTGCCCACAGCC
p-IFN-β-F GCTAACAAGTGCATCCTCCAAA
p-IFN-β-R AGCACATCATAGCTCATGGAAAGA
p-GAPDH-F ACATGGCCTCCAAGGAGTAAGA
p-GAPDH-R GATCGAGTTGGGGCTGTGACT