Table 2.
Identification of differentially expressed cellular proteins in PRRSV-infected Marc-145 cells and PAMs.
| Spot numbera | Protein name | Accession numberb | MW (Da) | pI | Protein score | Sequence coverage (%) |
|---|---|---|---|---|---|---|
| Significantly up-regulated proteins | ||||||
| Cytoskeletal protein | ||||||
| M3 | Cofilin-1 | gi|73960624 | 25,773 | 6.93 | 248 | 82 |
| P3 | Actin-related protein | gi|62510460 | 16,278.3 | 5.47 | 127 | 50 |
| P15 | Vimentin | gi|418884 | 30,826.5 | 6.47 | 58 | 40 |
| P16 | Alpha-cardiac actin | gi|553859 | 16,758.2 | 5.29 | 128 | 30 |
| P20 | Cofilin-1 | gi|51592135 | 18,506.6 | 8.16 | 156 | 18 |
| Response to stress/anti-oxidative stress proteins | ||||||
| M6 | Stress-70 protein | gi|73970890 | 55,119.2 | 5.12 | 94 | 14 |
| M11 | Peroxiredoxin-2 | gi|55727787 | 19,418 | 5.38 | 266 | 38 |
| M20 | Heat shock 27 kDa protein 1 | gi|55926209 | 22,927.7 | 6.23 | 177 | 28 |
| M19 | Peroxiredoxin-6 | gi|84579335 | 24,995.1 | 5.74 | 184 | 51 |
| P13 | Heat shock protein beta-1 (HSPB1) | gi|4504517 | 22,768.5 | 5.98 | 190 | 42 |
| Ubiquitin–proteosome pathway | ||||||
| M13 | Ubiquitin | gi|51701919 | 8559.6 | 6.56 | 152 | |
| Metabolic process | ||||||
| P2 | Cystatin-B (CSTB) | gi|76608397 | 25,288.5 | 6.44 | 97 | 31 |
| P4 | FYVE finger-containing phosphoinositide kinase | gi|6685475 | 232,904.3 | 6.34 | 77 | 20 |
| P22 | Pyruvate kinase isozymes M1/M2 (Pkm 2) | gi|206205 | 57,744 | 7.15 | 279 | 40 |
| Others | ||||||
| M10 | UPF 0681 protein KIAA1033 | gi|85397087 | 136,330.2 | 7.1 | 71 | 21 |
| P10 | Tropomyosin alpha-4 chain (TPM4) | gi|4507651 | 28,504.5 | 4.67 | 288 | 60 |
| P21 | UPF 0568 protein | gi|78369460 | 28,191.7 | 6.19 | 111 | 35 |
| Significantly down-regulated proteins | ||||||
| Cytoskeletal protein | ||||||
| M5 | LIM and SH3 protein 1 | gi|6754508 | 29,975.3 | 6.61 | 97 | 41 |
| M7 | Plectin-1 | gi|73974726 | 532,578.5 | 5.72 | 75 | 8 |
| M21 | Glial fibrillary acidic protein | gi|14193690 | 46,497.7 | 5.05 | 60 | 35 |
| P6 | Plectin-1 | gi|73974724 | 516,572.1 | 5.6 | 111 | 24 |
| Signaling transduction | ||||||
| M22 | Galectin-1 | gi|84027796 | 14,736.2 | 5.65 | 78 | 10 |
| P18 | Galectin-1 | gi|47716872 | 14,590.2 | 5.07 | 149 | 34 |
| Response to stress/anti-oxidative stress proteins | ||||||
| P9 | Superoxide dismutase 1 (SOD1) | gi|15082144 | 15,236.6 | 6.04 | 201 | 61 |
| Metabolic process | ||||||
| M1 | Prohibitin | gi|67970515 | 29,757.9 | 5.57 | 365 | 56 |
| M15 | Prohibitin | gi|67970515 | 29,757.9 | 5.57 | 365 | 56 |
| P7 | Epidermal fatty acid-binding protein 5 (Fabp5) | gi|89886167 | 15,199.5 | 6.6 | 128 | 30 |
| P8 | A-kinase anchoring protein AKAP350 | gi|4558862 | 416,855.1 | 4.94 | 85 | 18 |
| P17 | Pyridoxine 5′-phosphate oxidase variant | gi|62898045 | 29,896.8 | 7.01 | 137 | 16 |
P: protein of porcine alveolar macrophages (PAMs). M: protein of Marc-145 cells.
a
Refers to the labels of protein spots in Fig. 1.
b
Accession number that was obtained through the input of MALDI-TOF MS/MS experimental results in a MASCOT search of the NCBInr database.