Table 4.
Triad determinants of ALOX15 orthologs and positional specificity of arachidonic acid oxygenation. The one letter code of amino acid nomenclature was used and the triad determinants are indicated in bold face. In human ALOX15 amino acid numbering is as follows: Borngräber 1 determinant (F353), Sloane determinants (I418, M419), Borngräber 2 determinants (I593, T594). n.d., not yet determined experimentally.
| Species | Borngräber 1 determinant | Sloane determinant | Borngräber 2 determinant | Major product (predicted) | Major product (experimental) |
|---|---|---|---|---|---|
| Human (Homo sapiens) | DFQ | QIMS | SITW | 15-HpETE | 15-HpETE |
| Chimpanzee (Pan troglodytes) | DFQ | QIMS | SITW | 15-HpETE | n.d |
| Gorilla (Gorilla gorilla) | DFG | QIMS | SITW | 15-HpETE | n.d. |
| Orangutan (Pongo pygmaeus/abelii) | DFQ | QIMS | SITW | 15-HpETE | 15-HpETE |
| Gibbon (Nomascus leucogenys) | DFQ | QITS | SIVW | 12- + 15-HpETE | n.d. |
| Rhesus monkey (Macaca mulatta) | DFQ | QVVS | SITW | 12-HpETE | 12-HpETE |
| Baboon (Papio anubis) | DFQ | QVVS | SMTW | 12-HpETE | n.d. |
| Cattle (Bos taurus) | DFQ | QVVS | SITW | 12-HpETE | 12-HpETE |
| Pig (Sus scrofa) | DFQ | QVVS | SITW | 12-HpETE | 12-HpETE |
| Rat (Rattus norvegicus) | DLQ | KAMS | NVVW | 12-HpETE | 12-HpETE |
| Mouse (Mus musculus) | DLQ | KVMS | NVVW | 12-HpETE | 12-HpETE |