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. 2010 Jun 22;137(3):251–260. doi: 10.1016/j.vetimm.2010.06.001

Fig. 1.

Fig. 1

Characterization of recombinant α-enolase and polyclonal anti-α-enolase IgY antibodies. Samples in each panel are protein markers (lane M), purified GST (lane 1), purified α-enolase (lane 2), and purified GST-α-enolase fusion protein (lane 3). Purified proteins visualized by Coomassie blue staining (panel A) were blotted onto nitrocellulose paper and probed with anti-GST antibodies (panel B) or sera from 4th-immunized chicken (panel C). The molecular weight of recombinant α-enolase protein is about 48 kD.